Staying Current The polymerase chain reaction

Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abuse...

Uterine Leiomyoma and Reproductive Tract Infections Detected by Polymerase Chain Reaction

Background and Objective: For nearly a century, it has been suspected that reproductive tract infections play an etiologic role in uterine leiomyoma. However, no epidemiologic study of leiomyoma has used the polymerase chain reaction (PCR) to compare uterine tissues from cases and non-cases, and to investigate associations between uterine leiomyoma and infections detected by PC...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Comparison of fine needle aspiration and direct skin smear in the diagnosis of acute cutaneous leishmaniasis: polymerase chain reaction as a reference method

Background: Accurate diagnosis of cutaneous leishmaniasis wouldavoid unnecessary treatment and scar formation. Direct smear isthe most common method for the diagnosis of this disease but itsnegative result could not rule out the infection; so, the need formore sensitive methods is obvious. We conducted this study tocompare the efficiency of direct skin smear with smears preparedby fine needle a...

Polymerase Chain Reaction Study of Anogenital Condylomas for High Risk HPV Types

Background: Anogenital wart is a common sexually transmitted disease affecting both genders. The aim of this study was to evaluate human papilloma virus types of anogenital warts with Polymerase Chain Reaction (PCR). Method: 65 formalin fixed paraffin embedded (FFPE) tissue blocks of the anogenital warts obtained from the pathology lab of Loghman Hakim hospital were included in this study. Resu...